Metabolism of Specifically Labeled Glucose, Glucose 1-Phosphate, and Glucose 6-Phosphate via the Oxidative Pentose Phosphate Cycle in a Reconstituted Spinach Chloroplast System in Darkness and in the Light |
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Authors: | Lendzian K J |
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Institution: | Institut für Botanik und Mikrobiologie, Lehrstuhl für Botanik, Technische Universität, D-8000 München 2, Federal Republic of Germany |
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Abstract: | Using isolated spinach (hybrid 424) chloroplasts deprived of their envelopes (reconstituted chloroplast system), the metabolism of glucose, glucose 1-phosphate, and glucose 6-phosphate via the oxidative pentose phosphate cycle was analyzed. The activity of oxidative pentose phosphate cycle was monitored by continuous sampling of the CO2 released during the decarboxylation process of 6-phosphogluconate. |
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