Quantitative three-dimensional study on the position of the female gametophyte and its constituent cells as a prerequisite for corn (Zea mays) transformation |
| |
| Authors: | V T Wagner C Dumas H L Mogensen |
| |
| Institution: | (1) Reconnaissance Cellulaire et Amelioration des Plantes, Université Claude Bernard-Lyon I/La INRA 879 43 Boul. du 11 Novembre 1918, F-69622 Villeurbanne Cedex, France;(2) Department of Biological Sciences, Northern Arizona University, Box 5640, 86011 Flagstaff, AZ, USA;(3) Present address: Dipartimento di Biologia Ambientale, Universita di Siena, Via Mattioli 4, I-53100 Siena, Italy |
| |
| Abstract: | Summary The position of the embryo sac in the spikelet and of the embryo sac's constituent cells within the sporophytic tissues of Zea mays was localized by scanning electron microscopy, serial thick sectioning, and computer three-dimensional reconstruction. Within certain limits, the embryo sac is consistently oriented in the same position inside of the spikelet. This information is a prerequisite for successful microinjections into the in situ female cells of Zea mays. |
| |
| Keywords: | Biotechnology Embryo sac Microinjection Micromanipulation |
| 本文献已被 SpringerLink 等数据库收录！ |
|
