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High-performance liquid chromatography for assaying NAD glycohydrolase from Neurospora crassa conidia |
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| Authors: | P Pietta M Pace F Menegus |
| Institution: | 1. Istituto di Chimica Organica ed Analitica, University of Milano, via G. Celoria, 2, 20133 Milano, Italy;2. Laboratorio di Biosintesi Vegetale, CNR, Via E. Bassini 15, 20133 Milano, Italy |
| Abstract: | A rapid and sensitive high-performance liquid chromatographic technique was developed to determinate NAD glycohydrolase (EC 3.2.2.5.) activity from Neurospora crassa conidia. The separation of the assay substrate and products was achieved by isocratic reverse-phase chromatography and the peaks were detected by the absorbance at 259 nm. Quantities of NAD+ and nicotinamide as small as 10 pmol could be measured. |
| Keywords: | high-performance liquid chromatography NAD glycohydrolase NADase To whom correspondence should be addressed |
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