Molecular phylogenetic analysis ofVibrio cholerae O1 El Tor strains isolated before, during and after the O139 outbreak based on the intergenomic heterogeneity of the 16S-23S rRNA intergenic spacer regions |
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Authors: | Atreyi Ghatak Anasuya Majumdar Ranajit K Ghosh |
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Institution: | (1) Molecular Biology Laboratory, Indian Institute of Chemical Biology, 4, Raja SC Mullick Road Jadavpur, 700 032 Kolkata, India |
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Abstract: | We have cloned, sequenced and analysed all the five classes of the intergenic (16S-23S rRNA) spacer region (ISR) associated
with the eightrrn operons (rrna-rrnh) ofVibrio cholerae serogroup O1 El Tor strains isolated before, during and after the O139 outbreak. ISR classes ‘a’ and ‘g’ were found to be
invariant, ISR-B (ISRb and ISRe) exhibited very little variation, whereas ISR-C (ISRc, ISRd, and ISRf) and ISRh showed the
maximum variation. Phylogenetic analysis conducted with all three ISR classes (ISR-B, ISR-C and ISRh) showed that the pre-O139
serogroup and post-O139 serogroup O1 El Tor strains arose out of two independent clones, which was congruent with the observation
made by earlier workers suggesting that analyses of ISR-C and ISR-h, instead of all five ISR classes, could be successfully
used to study phylogeny in this organism. |
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Keywords: | Intergenic spacer region (ISR) phylogeny Vibrio cholerae |
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