Kinetic characterization of the acyl-enzyme mechanism for beta-lactamase I. |
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| Authors: | M T Martin and S G Waley |
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| Affiliation: | Sir William Dunn School of Pathology, University of Oxford, U.K. |
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| Abstract: | beta-Lactamase I catalyses the hydrolysis of penicillins by an acyl-enzyme mechanism. A procedure was developed for determining the rate constants for the acylation and deacylation steps for the good substrates benzylpenicillin and phenoxymethylpenicillin; this depends on determining the fraction of enzyme that is present as acyl-enzyme in the steady state. |
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