Superoxide dismutase in vesicular arbuscular-mycorrhizal red clover plants |
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| Authors: | José M Palma M Angeles Longa Luis A del Río Justo Arines |
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| Institution: | Unidad de Fisiolo-gia Vegetal. Institute de Investigaciones Agrobiologicas de Galicia. CSIC. Apdo 122, E-15080 Santiago de Compostela. Spain;Unidad de Bioquimica Vegetal, Estación Experimental del Zaidin, CSIC, Apdo 419, E-18080 Granada. Spain. |
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| Abstract: | The isoenzymatic pattern of Superoxide dismutase (SOD; EC 1.15.1.1) was studied in the symbiosis of Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe-Trifolium prarense L A Cu.Zn-SOD (M, 40500) was found in spores of G. mosseae . while one Mn-SOD (Mn-SOD I) and two Cu.Zn-SODs (Cu.Zn-SOD 1 and Cu.Zn-SOD II) were present in both roots and leaves of T. pratense . Molecular masses for Cu.Zn-SOD I and Cu.Zn-SOD II were 31000 and 34300. respectively. However, when T. prateme and G. mosseae were associated, mycorrhizal roots showed two new iso-zymes, Mn-SOD II and mycCu.Zn-SOD, which have relative molecular masses of 37 800 and 33 300, respectively. The mycCu.Zn-SOD was found to be specific for this association, whereas Mn-SOD II was also present in nodules of Rhizobium-T. pra-tense . Results suggest that both enzymes are induced in the T. praiense roots in response to invasion by mycorrhizal fungi, perhaps as a result of an increase in the generation of O-2 radicals in plant roots. |
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| Keywords: | Glomus mosseae induction isozymes mycorrhizae red clover Rhizobium-nodules Superoxide dismutase Trifolium prateme VAM fungi |
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