In silico analysis of chimeric espA, eae and tir fragments of Escherichia coli O157:H7 for oral immunogenic applications |
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| Authors: | Jafar Amani S Latif Mousavi Sima Rafati Ali H Salmanian |
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| Institution: | (1) National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrak-e- Pajoohesh, 15th Km, Tehran -Karaj Highway, Tehran, IR, Iran;(2) Department of Biotechnology, Baqiyatallah University of Medical Science, Tehran, IR, Iran;(3) Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, IR, Iran;(4) Molecular Immunology and Vaccine Research Laboratory, Department of Immunology, Pasteur Institute of Iran, Tehran, IR, Iran |
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| Abstract: | Background
In silico techniques are highly suited for both the discovery of new and development of existing vaccines. Enterohemorrhagic Escherichia coli O157:H7 (EHEC) exhibits a pattern of localized adherence to host cells, with the formation of microcolonies, and induces
a specific histopathological lesion (attaching/effacing). The genes encoding the products responsible for this phenotype are
clustered on a 35-kb pathogenicity island. Among these proteins, Intimin, Tir, and EspA, which are expressed by attaching-effacing
genes, are responsible for the attachment to epithelial cell that leads to lesions. |
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| Keywords: | |
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