Development of consistently crossable wheat genotypes for alien wheat gene transfer through fine-mapping of the <Emphasis Type="Italic">Kr1</Emphasis> locus |
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Authors: | Isabelle Bertin Lesley Fish Tracie N Foote Emilie Knight John Snape Graham Moore |
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Institution: | 1.Department of Genetics, Diversity and Ecophysiology of Cereals,UMR INRA-UBP,Clermont-Ferrand,France;2.Department of Crop Genetics,John Innes Centre,Norwich,UK |
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Abstract: | Breeders can force sexual hybridisation between wheat and related grass species to produce interspecific hybrids containing
a dihaploid set of wheat and related chromosomes. This facilitates the introgression of desirable genes into wheat from the
secondary gene pool. However, most elite European wheat varieties carry genes that suppress crossability, making the transfer
of novel traits from exotic germplasm into elite wheat varieties difficult or impossible. Previous studies have identified
at least five crossability loci in wheat. Here, the crossability locus with the largest effect, Kr1 on chromosome arm 5BL, was fine-mapped by developing a series of recombinant substitution lines in which the genome of the
normally non-crossable wheat variety ‘Hobbit sib’ carries a recombinant 5BL chromosome arm containing segments from the crossable
variety ‘Chinese Spring’. These recombinant lines were scored for their ability to cross with rye over four seasons. Analysis
revealed at least two regions on 5BL affecting crossability, including the Kr1 locus. However, the ability to set seed is highly dependent on prevailing environmental conditions. Typically, even crossable
wheat lines exhibit little or no seed set when crossed with rye in winter, but show up to 90% seed set from similar crosses
made in summer. By recombining different combinations of the two regions affecting crossability, wheat lines that consistently
exhibit up to 50% seed set, whether crossed in the UK winter or summer conditions, were generated, thus creating a very important
tool for increasing the efficiency of alien wheat transfer programmes. |
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