Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations |
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Authors: | Frank Kensy Christoph Engelbrecht and Jochen Büchs |
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Institution: | (1) AVT-Biochemical Engineering, Sammelbau Biologie, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany;(2) m2p-labs GmbH, Forckenbeckstra?e 6, 52074 Aachen, Germany |
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Abstract: | Background In the past decade, an enormous number of new bioprocesses have evolved in the biotechnology industry. These bioprocesses
have to be developed fast and at a maximum productivity. Up to now, only few microbioreactors were developed to fulfill these
demands and to facilitate sample processing. One predominant reaction platform is the shaken microtiter plate (MTP), which
provides high-throughput at minimal expenses in time, money and work effort. By taking advantage of this simple and efficient
microbioreactor array, a new online monitoring technique for biomass and fluorescence, called BioLector, has been recently
developed. The combination of high-throughput and high information content makes the BioLector a very powerful tool in bioprocess
development. Nevertheless, the scalabilty of results from the micro-scale to laboratory or even larger scales is very important
for short development times. Therefore, engineering parameters regarding the reactor design and its operation conditions play
an important role even on a micro-scale. In order to evaluate the scale-up from a microtiter plate scale (200 μL) to a stirred
tank fermenter scale (1.4 L), two standard microbial expression systems, Escherichia coli and Hansenula polymorpha, were fermented in parallel at both scales and compared with regard to the biomass and protein formation. |
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