Atypical fast SERCA1a protein expression in slow myofibers and differential S-nitrosylation prevented by exercise during long term bed rest |
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Authors: | Michele Salanova Gudrun Schiffl Dieter Blottner |
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Institution: | 1.Department of Vegetative Anatomy, Center of Space Medicine Berlin, Neuromuscular Group,Charité Universit?tsmedizin Berlin,Berlin,Germany |
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Abstract: | We monitored changes in SERCA isoform specific expression and S-nitrosylation in myofibers of lower limb soleus (SOL) and
vastus lateralis (VL) muscle biopsies before and after 60 days of voluntary long term bed rest (BR) without (BR-CTRL group,
n = 8) and with exercise countermeasure (BR-EX group, n = 8). Before BR, a typical myofiber type-specific distribution of fast and slow SERCA1/2a isoforms was seen. After BR, a
subpopulation (approx. 15%) of slow myofibers in BR-CTRL additionally expressed the fast SERCA1a isoform which was not seen
in BR-EX. After BR, SERCA1a S-nitrosylation patterns analyzed by the biotin-switch assay decreased in disused SOL only but
increased in both muscles following exercise. Differential SERCA1a S-nitrosylation and SERCA1a/2a co-expression in subsets
of slow myofibers should be considered as signs of an altered cytosolic Ca2+ homeostasis following chronic muscle disuse. Exercise preserved myofiber type-specific SERCA1a expression and S-nitrosylation
in VL and SOL in a different way, suggesting muscle-specific responses to the countermeasure protocol applied during bed rest. |
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