Plant regeneration from protoplasts of Capsicum annuum |
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| Authors: | I Díaz R Moreno J B Power |
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| Institution: | (1) UEI Fitopatología, Centro de Investigaciones Biológicas, CSIC, c/Velazquez 144, 28006 Madrid, Spain;(2) Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD Nottingham, UK;(3) Present address: Centro de Investigación, CEPSA c/Picos de Europa 6, Poligono, Industrial S. Fernándo de Henares 2, Madrid, Spain |
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| Abstract: | Leaf protoplasts were isolated from axenic shoot cultures of four varieties of Capsicum annuum (Americano, Dulce Italiano Florida Gynat and Nigrum) and a wild species C. chinense. Protoplasts of both species, cultured in KM8P medium and using agarose bead culture, entered division with the exception of the variety Nigrum. Cell colonies formed callus in agar-solified MS medium supplemented with zeatin and for C. annuum v. Dulce Italiano shoots were regenerated when protoplast-derived calli were transferred to MS medium with 6-BAP. Excised shoots were rooted on MS medium which lacked phytohormones.Abbreviations 6-BAP
6-benzylamino purine
- MS
Murashige and Skoog (1962)
- KM8P
Kao and Michayluk (1975)
- SDS
sodium dodecyl sulphate |
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