The cross channel activities of spider neurotoxin huwentoxin-I on rat dorsal root ganglion neurons |
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Authors: | Wang Meichi Guan Xin Liang Songping |
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Affiliation: | Key Laboratory of Protein Chemistry and Developmental Biology of Education, The College of Life Science, Hunan Normal University, Changsha 410081, China. |
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Abstract: | In this paper, we investigated the action of huwentoxin-I (HWTX-I) purified from the venom of the Chinese bird spider Ornithoctonus huwena on Ca(2+), Na(+) channels of adult rat dorsal root ganglion (DRG) neurons. The results showed that huwentoxin-I could reduce the peak currents of N-type Ca(2+) channels (IC(50) approximately 100 nM) and TTX-S Na(+) channels (IC(50) approximately 55 nM), whereas no effect was detected on TTX-R Na(+) channels. The comparative studies indicated that the selectivity of HWTX-I on Ca(2+) channels was higher that of MVIIA and approximately the same as that of GVIA. HWTX-I is the first discovered toxin with the cross channel activities from the spider O. huwena venom similar to micro O-conotoxins MrVIA and MrVIB. |
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Keywords: | HWTX-I, huwentoxin-I DRG, dorsal root ganglion VGCCs, voltage-gated calcium channel VGSC, voltage-gated sodium channel TTX-R, tetrodotoxin-resistent TTX-S, tetrodotoxin-sensitive rat |
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