Fatty Acid and Polar Lipid Composition in the Classification of Kurthia |
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Authors: | M GOODFELLOW M D COLLINS D E MINNIKIN |
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Institution: | Department of Microbiology, The University, Newcastle upon Tyne NE1 7RU, U.K.;Department of Organic Chemistry, The University, Newcastle upon Tyne NE1 7RU, U.K. |
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Abstract: | Strains of Kurthia zopfii were degraded by acid methanolysis and the non-hydroxylated fatty acid esters so released were examined by gas liquid chromatography. The major fatty acid types were straight-chain, anteiso - and iso -methyl branched-chain acids. Monounsaturated fatty acids were not detected. The major fatty acid in five of the six strains examined consisted of 12-methyltetra-decanoic ( anteiso -C15) acid. The other strain possessed major amounts of both 13-methyltetradecanoic ( iso -C15) and anteiso -C15 acids. Polar lipids of all the strains were examined by two-dimensional thin-layer chromatography. All possessed a very simple polar lipid composition consisting of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. |
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