Decrease in the levels of a constitutive cytochrome P-450 (RLM5) in hepatic microsomes of diabetic rats |
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| Authors: | L V Favreau J B Schenkman |
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| Institution: | 1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China;2. Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China;1. Sanquin Research, Amsterdam, The Netherlands;2. Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, The Netherlands;3. Sanquin Diagnostic Services, Amsterdam, The Netherlands;4. Jan van Breemen Research Institute/Reade, Amsterdam, The Netherlands |
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| Abstract: | Cytochrome P-450 dependent hydroxylation of testosterone has been measured in hepatic microsomes of control, diabetic and insulin-treated diabetic rats. The observed decrease in testosterone 16 alpha-hydroxylase activity in diabetes, an activity previously shown to be largely due to RLM5, was accompanied by a dramatic decrease in immunodetectable RLM5. Diabetic rats which received insulin had elevated testosterone 16 alpha-hydroxylase activity relative to the diabetic animals, which was accompanied by a corresponding increase in the levels of RLM5. These results provide evidence that specific constitutive cytochrome P-450 enzymes are altered in the diabetic state and that these changes are not permanent since they can be overcome, at least partially, by insulin replacement therapy. |
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