Functional antagonism by a monoclonal antibody to digoxin in a test system of cultured rat heart myocytes |
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| Authors: | Wallukat G. Simon H. -U. Müller W. -D. Wolf I. |
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| Affiliation: | (1) Max Delbrück Centre for Molecular Medicine, Robert-Rössle-Str. 10, 13125 Berlin-Buch, Germany;(2) Institute of Clinical Immunology, Friedrich-Schiller Universität Jena, Am Johannesfriedhof 3, 07740 Jena, Germany |
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| Abstract: | A monoclonal antibody with a high affinity for digitoxin (KA = 0.50 nM) and digoxin (KA = 0.55 nM) was produced by somatic cell fusion. This antibody, designated 2A3(47), displayed little cross reactivity with other glycosides. In cultured rat heart myocytes, 2A3(47), antagonized the positive chronotropic effect exerted by digitoxin but did not alter that of ouabain. Our results suggest that this monoclonal antibody may prove to be useful in treating digoxin and digitoxin intoxication. |
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| Keywords: | glycosides monoclonal antibodies cardiac myocytes cell culture digitoxin intoxication |
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