M-DNA: A complex between divalent metal ions and DNA which behaves as a molecular wire |
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| Authors: | Aich P Labiuk S L Tari L W Delbaere L J Roesler W J Falk K J Steer R P Lee J S |
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| Affiliation: | Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada. |
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| Abstract: | M-DNA is a complex of DNA with divalent metal ions (Zn(2+), Co(2+), or Ni(2+)) which forms at pH conditions above 8. Upon addition of these metal ions to B-DNA at pH 8.5, the pH decreases such that one proton is released per base-pair per metal ion. Together with previous NMR data, this result demonstrated that the imino proton in each base-pair of the duplex was substituted by a metal ion and that M-DNA might possess unusual conductive properties. Duplexes of 20 base-pairs were constructed with fluorescein (donor) at one end and rhodamine (acceptor) at the other. Upon formation of M-DNA (with Zn(2+)) the fluorescence of the donor was 95 % quenched. Fluorescence lifetime measurements showed the presence of a very fast component in the decay kinetics with tau
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