Cell lines from imaginal discs ofDrosophila melanogaster |
| |
Authors: | K Ui R Ueda T Miyake |
| |
Institution: | (1) Laboratory of Cell Biology, Mitsubishi-Kasei Institute of Life Sciences, 11 Minamiooya, Machida-Shi, 194 Tokyo, Japan;(2) Present address: Graduate School of Science and Engineering, Waseda University, Japan |
| |
Abstract: | Summary New cell lines, designated as ML-DmDl≈10, were established from dissociated imaginal discs ofDrosophila melanogaster. The culture medium was prepared by mixing in a 1:1 ratio Cross and Sang’s M3(BF) medium, supplemented with 10% heat inactivated
fetal bovine serum (FBS), with the supernatant of a primary embryonic cell culture made in the M3(BF) medium and supplementing
this mixture with insulin. One cell line was established in the medium containing larval hemolymph instead of the primary
culture supernatan, and another was established in fresh M3(BF) medium supplemented with insulin and FBS. In these mediums,
imaginal disc cells first formed aggregates and cellular vesicles within a few weeks followed by the proliferation of thin-layered
cells around them after about 1 mo. Ten cell lines have so far been established from two kinds of imaginal discs and disc
mixtures. The ploidy of these cell lines was predominantly diploid. Population doubling time was about 50 to 70 h at 3 to
10 mo. after initiation of the culture. When the cell aggregates formed in vitro were implanted in metamorphosing larvae,
they differentiated at high frequency into adult cuticular strutures in the early phase of the primary culture. This differentiation
of aggregates was also observed, though at low frequency, in a culture maintained by dilution-transfer for 6 to 15 mo. in
vitro. |
| |
Keywords: | Drosophila melanogaster imaginal disc conditioned medium cell line insulin |
本文献已被 SpringerLink 等数据库收录! |
|