Laccase is upregulated via stress pathways in the phytopathogenic fungus Sclerotinia sclerotiorum |
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Authors: | Cristina Coman Augustin C Mo? Emese Gal Marcel Pârvu Radu Silaghi-Dumitrescu |
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Institution: | 1. Department of Chemistry and Chemical Engineering, ‘Babe?-Bolyai’ University, 1, M. Kog?lniceanu Street, Cluj-Napoca RO-400084, Romania;2. Department of Biology and Geology, ‘Babe?-Bolyai’ University, 1, M. Kog?lniceanu Street, Cluj-Napoca RO-400084, Romania |
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Abstract: | We report on the factors affecting the production of the newly characterized laccase from the phytopathogenic fungus Sclerotinia sclerotiorum (Lib.) de Bary. The carbon/nitrogen ratio appears to be of great importance. Rather than a simple nutrient-rich nitrogen source, yeast extract (YE) behaves as a true laccase upregulator, apparently acting via a stress pathway. Chelidonium majus extract, a known antifungal agent, acts in a similar manner. The compound(s) in the YE responsible for enhancing laccase synthesis are suggested to be hydrolysable choline derivatives. Both extracts reduce biomass and sclerotia development and enhance laccase production, leading to an increase in laccase activity by one order of magnitude compared to controls. The pH of the medium, a well-known virulence regulator for this fungus, also acts as a true laccase regulator, though via a different mechanism. The effect of pH appeared to be linked to the acidification kinetics of the extracellular medium during fungal development. A number of other known laccase inducers were found to enhance laccase production at most twofold. |
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Keywords: | Culture optimization Laccase regulation pH Stress response Yeast extract |
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