Replication of mini-F plasmid in vitro promoted by purified E protein |
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| Authors: | Kanae Muraiso Takashi Tokino Tomoaki Murotsu Kenichi Matsubara |
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| Affiliation: | (1) Institute for Molecular and Cellular Biology, Osaka University, 565 Suita, Osaka, Japan |
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| Abstract: | Summary An in vitro system for replication of mini-F plasmid DNA was constructed. This system consists of an ammonium sulfate fraction II (Fuller et al. 1981) from Escherichia coli extract, exogeneously added purified E protein encoded by mini-F plasmid, and mini-F DNA in a closed circular form. Experiments with this system showed that the 217 bp DNA region which contains the A+T rich cluster and the four 19 bp direct repeats responsible for incB incompatibility is essential for mini-F DNA replication. |
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| Keywords: | Plasmid mini-F in vitro replication E protein |
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