Isolation and characterization of enterocin EJ97, a bacteriocin produced by Enterococcus faecalis EJ97 |
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| Authors: | A. Gálvez E. Valdivia Hikmate Abriouel Emilio Camafeita Enrique Mendez Manuel Martínez-Bueno Mercedes Maqueda |
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| Affiliation: | (1) Departamento de Microbiología, Facultad de Ciencias Experimentales, Universidad de Jaén, E-23071 Jaén, Spain e-mail: agalvez@ujaen.es. Tel. +53-212160; Fax +53-212141, ES;(2) Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Fuentenueva s/n, E-18071 Granada, Spain, ES;(3) Centro Nacional de Biotecnología, CSIC, Cantoblanco, E-28049-Madrid, Spain, ES;(4) Instituto de Biotecnología, Universidad de Granada, E-18071 Granada, Spain, ES |
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| Abstract: | The bacteriocinogenic strain of Enterococcus faecalis EJ97 has been isolated from municipal waste water. It produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,340 Da) that is very stable under mild heat conditions and is sensitive to proteolytic enzymes. The amino acid sequence of the first 18 N-terminal residues of enterocin EJ97 indicates that it is different from other known protein sequences. Enterocin EJ97 is active on several gram-positive bacteria including enterococci, several species of Bacillus, Listeria, and Staphylococcus aureus. The producer strain is immune to bacteriocin. Enterocin EJ97 has a concentration-dependent bactericidal and bacteriolytic effect on E. faecalis S-47. Received: 15 July 1998 / Accepted 28 October 1998 |
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| Keywords: | Bacteriocin Enterococcus Listeria Cationic peptide |
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