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cHS4 insulator-mediated alleviation of promoter interference during cell-based expression of tandemly associated transgenes |
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| Authors: | Yahata Kazuhide Maeshima Kazuhiro Sone Takefumi Ando Taichi Okabe Masaru Imamoto Naoko Imamoto Fumio |
| Institution: | 1 Department of Molecular Biology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 2 Cellular Dynamics Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198 Japan 3 Department of Experimental Genome Research, Faculty of Pharmaceutical Sciences, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan |
| Abstract: | |
| Keywords: | cHS4 chicken β-globin DNaseI hypersensitive site 4 CTCF CCCTC-binding factor CBP CREB-binding protein DAPI 4&prime 6-diamidino-2-phenylindole Flp flipase FRT Flp recognition targets EF-1α elongation factor-1α HAT histone acetyltransferase mRFP1 monomeric red fluorescent protein SECFP super enhanced cyan fluorescent protein siRNA small interfering RNA USF1 upstream stimulatory factor 1 Venus super enhanced yellow fluorescent protein |
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