Interactions between extracellular matrix components and proteoglycans released from monocytesin vitro |
| |
Authors: | Svein O Kolset |
| |
Institution: | (1) Department of Tumor Biology, Norwegian Cancer Society, Institute of Medical Biology, University of Tromsø, Tromsø, Norway |
| |
Abstract: | 35S-labelled chondroitin sulfate proteoglycans isolated from conditioned media of cultured human monocytes (day 1in vitro) and monocyte-derived macrophages (day 6in vitro) were chromatographed on columns of immobilized fibronectin and collagen, respectively. The elution profiles prior to and after alkali treatment were compared with those of standards chondroitin 4-sulfate and chondroitin sulfate E and heparin. The day 635S-proteoglycans have a higher sulfate density than the day 1 species, but this difference did not affect the elution profiles after chromatography on collagen-Sepharose, whereas the day 6 proteoglycans bound more firmly than the day 1 fraction to fibronectin-Sepharose. The elution patterns obtained for these distinct proteoglycans closely resembled those of heparin and oversulfated chondroitin sulfate E standards, and clearly demonstrated the importance of sulfate density both for the affinity to fibronectin and collagen. Neither day 1 nor day 635S-proteoglycans were found to interact with hyaluronate.Abbreviations used CSPG
chondroitin sulfate proteoglycan
- GAG
glycosaminoglycan
- CS
chondroitin sulfate
- CS-E
chondroitin 4,6 disulfate
- MDM
monocyte-derived macrophages |
| |
Keywords: | Chondroitin sulfate proteoglycan affinity to collagen fibronectin hyaluronate monocytes |
本文献已被 SpringerLink 等数据库收录! |
|