Anti-tumour components of the culture filtrates from Tricholoma sp. |
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Authors: | F Liu V E C Ooi S T Chang |
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Institution: | (1) Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong |
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Abstract: | The anti-tumour component (Fraction A) found in the culture filtrates of a local mushroom, Tricholoma sp. (strain STC20-T1), was purified using DEAE-cellulose ion exchange chromatography and Sepharose CL-4B gel filtration. Of its two purified sub-fractions, A-1 and A-2, only the latter showed anti-tumor activity. When Fraction A-2 was treated with cetyltrimethylammonium hydroxide and chloroform/butanol (24:1, v/v), it behaved as a proteinbound polysaccharide, with a molecular mass estimated to be 154 kDa by gel filtration. It contained 40% polysaccharide, which consisted of fucose, galactose, glucose, arabinose and mannose, and 30.5% protein, the amino-acid composition of which was determined. Fraction A-2 could inhibit the growth of Sarcoma-180 implanted in mice intraperitoneally and subcutaneously, with no sign of toxicity, at a dose of 20 mg/kg.day for 10 days. |
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Keywords: | Anti-tumour activity polysaccharide Sarcoma-180 (S-180) Tricholoma sp |
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