| 地衣芽孢杆菌普鲁兰酶编码基因的鉴定 |
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| 引用本文： | 张明焱,沈微,饶志明,方慧英,诸葛斌,诸葛健.地衣芽孢杆菌普鲁兰酶编码基因的鉴定[J].生物学杂志,2009,26(2):8-10. |
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| 作者姓名： | 张明焱 沈微 饶志明 方慧英 诸葛斌 诸葛健 |
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| 作者单位： | 江南大学,工业生物技术教育部重点实验室,无锡,214122 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 对地衣芽孢杆菌基因组序列分析显示。其中标注为amyX的基因可能编码普鲁兰酶。以PCR方法，从地衣芽孢杆菌染色体DNA中扩增出amyX基因蛋白编码区，插入大肠杆菌表达载体pET28aT7启动予下游。含重组质粒的大肠杆菌BL21（DE3）在IPTG诱导下表达出有活性的普鲁兰酶。酶学性质初步分析表明，重组普鲁兰酶最适反应温度为40℃，最适pH值为6．0。
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| 关 键 词： | 普鲁兰酶 地衣芽孢杆菌 amyX |
Identification of a gene encoding pullulanase from Bacillus licheniformis |
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| ZHANG Ming-yan,SHEN Wei,RAO Zhi-ming,FANG Hui-ying,ZHUGE Bing,ZHUGE Jian.Identification of a gene encoding pullulanase from Bacillus licheniformis[J].Journal of Biology,2009,26(2):8-10. |
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| Authors: | ZHANG Ming-yan SHEN Wei RAO Zhi-ming FANG Hui-ying ZHUGE Bing ZHUGE Jian |
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| Institution: | ZHANG Ming-yan,SHEN Wei,RAO Zhi-ming,FANG Hui-ying,ZHUGE Bing,ZHUGE Jian(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214121,China) |
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| Abstract: | Analysis of the complete genome sequence of Bacillus licheniformis revealed a putative pullulanase gene annotated as amyX.The coding region of amyX gene was amplified from genome DNA of B.licheniformis by PCR and inserted into the downstream of T7 promoter of the expression vector pET28a.The recombinant plasmid was transformed into the Escherichia coli BL21(DE3).Induced with IPTG the recombinant E.coli expressed the pullulanase active.The recombinant enzyme activity showed optimal pH and temperature were 6.... |
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| Keywords: | amyX |
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