Somatic embryogenesis and plant regeneration in Lilium longiflorum Thunb |
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| Authors: | A Tribulato P C Remotti H J M Löffler J M van Tuyl |
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| Institution: | (1) Istituto di Orticoltura e Floricoltura, University of Catania, Via Valdisavoia, 5-95123, Catania, Italy, IT;(2) Enea C. R. Casaccia, INN-BIOAG-ECO, 00060 S. M. di Galeria Rome, Italy, IT;(3) Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Ornamental Crops, PO Box 16, 6700 AA-Wageningen, The Netherlands, NL |
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| Abstract: | Friable callus was obtained from styles and flower pedicels of Lilium longiflorum Snow Queen and the Oriental lily hybrid Star Gazer on Murashige and Skoog (MS) media containing either 2 μm dicamba or 2 μm picloram. Cell suspension cultures were established by suspending the callus of L. longiflorum Snow Queen in liquid medium containing 2 μm dicamba. Through a purification process, a fine fast-growing cell suspension was obtained. This suspension was composed of
a homogenous population of small dense cells, which tended to organise into embryo like structures (ELS). In liquid culture
with the auxin dicamba, the ELS underwent continuous callus formation. When transferred to solidified hormone-free MS medium,
the ELS germinated, forming complete plantlets. Histological investigation showed that in the ELS both shoot and root meristems
were distinctly evident. It was concluded that the ELS obtained were in fact somatic embryos.
Received: 4 April 1997 / Revision received: 13 May 1997 / Accepted: 15 June 1997 |
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| Keywords: | Lily Flower bulbs Somatic embryos Dicamba Picloram |
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