Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana |
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Authors: | Carrie Chris Murcha Monika W Kuehn Kristina Duncan Owen Barthet Michelle Smith Penelope M Eubel Holger Meyer Etienne Day David A Millar A Harvey Whelan James |
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Affiliation: | ARC Centre of Excellence in Plant Energy Biology, MCS Building M316 University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia. |
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Abstract: | We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria. |
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Keywords: | AOX, alternative oxidase GFP, green fluorescent protein KAT2, 3-ketoacyl-CoA thiolase ND, type II alternative NAD(P)H dehydrogenase RFP, red fluorescent protein SSU, Rubisco small subunit of ribulose 1,5 bisphosphate carboxylase/oxygenase TIM17-2, translocase of the inner mitochondrial membrane |
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