Nonrandom spatial distribution by mammalian cells in culture |
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Authors: | Philip Skehan Susan J Friedman |
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Institution: | (1) The Oncology Research Group, Department of Pharmacology, Faculty of Medicine, The University of Calgary, T2N 4N1 Calgary, Alberta, Canada |
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Abstract: | Quadrat analysis was used to investigate the spatial distribution of seven mammalian cell lines in culture. The number of
cells in replicate unit areas of the culture was determined, and the variance to mean ratio used as an index of random and
nonrandom spatial distribution. Only mouse SV3T3 cells distributed themselves randomly throughout the entire culture growth
cycle. The remaining six lines all assumed a nonrandom distribution at some point in their growth cycles. Mouse L929 cells
displayed avoidance behavior, and spaced themselves at regular intervals in a uniform spatial distribution. The five remaining
lines (mouse S180, rat C6, hamster CHO, canine MDCK, and human BeWo) formed multicellular clusters, and were distributed aggregatively
rather than randomly. Random walk migration can account for the random distribution of SV3T3 cells. Random walk combined with
contact inhibition of movement provides a satisfactory explanation for the uniform distribution of L929 cells. Random walk
and contact inhibition are incompatible with cell clustering, however. Thus other mechanisms of motility or adhesiveness must
contribute to cell clustering. It is possible that random walk and contact inhibition may be less common components of cell
movement than generally assumed. |
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Keywords: | Spatial distribution by mammalian cells in culture distribution spatial by mammalian cells in culture mammalian cells spatial distribution of nonrandom spatial distribution by mammalian cells |
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