TILLMore, a resource for the discovery of chemically induced mutants in barley |
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Authors: | Talamè Valentina Bovina Riccardo Sanguineti Maria Corinna Tuberosa Roberto Lundqvist Udda Salvi Silvio |
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Institution: | Department of Agroenvironmental Sciences and Technology, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy; Nordic Genetic Resource Center, PO Box 41, SE-230 53 Alnarp, Sweden |
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Abstract: | A sodium azide-mutagenized population of barley (cv. 'Morex') was developed and utilized to identify mutants at target genes using the 'targeting induced local lesions in genomes' (TILLING) procedure. Screening for mutations at four agronomically important genes (HvCO1, Rpg1, eIF4E and NR) identified a total of 22 new mutant alleles, equivalent to the extrapolated rate of one mutation every 374 kb. All mutations except one were G/C to A/T transitions and several (approximately 68%) implied a change in protein amino acid sequence and therefore a possible effect on phenotype. The high rate of mutation detected through TILLING is in keeping with the high frequency (32.7%) of variant phenotypes observed amongst the M(3) families. Our results indicate the feasibility of using this resource for both reverse and forward genetics approaches to investigate gene function in barley and related crops. |
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Keywords: | chemical mutagenesis forward genetics Hordeum vulgare reverse genetics TILLING |
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