一种简易小鼠尾部皮肤移植教学实验改良方法的建立

目的 为本校生物工程系实验动物学课程小鼠尾部皮肤移植实验课提供一种合适的操作方法.方法 分别用0.7%、1%、1.5%戊巴比妥钠和10%水合氯醛对小鼠进行麻醉试验,选出合适的麻醉方法;再进行小鼠尾部皮肤移植,对比玻璃套管法和创可贴法2种包扎方法的效果,以选出合适的手术方法.结果 0.7%和1%戊巴比妥钠麻醉持续时间过短,1.5%戊巴比妥钠和10%水合氯醛能维持足够手术操作所需的时间,但1.5%戊巴比妥钠易导致动物死亡,故采用10%水合氯醛麻醉小鼠较合适;玻璃套管法包扎的效果较创可贴法为好且更简便.结论 10%水合氯醛麻醉结合玻璃套管法包扎进行小鼠尾部皮肤移植是一种有效、简便、经济的小鼠尾部皮肤移植手术实验方法;此方法技术失败率低,适合学生实验课采用.

A Simple Mice Tail Skin Transplantation Improved Method for Experiment Teaching

Objective To establish a mice tail skin transplantation method for the experiment of laboratory animal science course in our college. Method The recipients were divided into four groups which received 0. 7% , 1% , 1.5% pentobarbital sodium and 10% chloral hydrate intraperitoneum injection respectively. And the other recipients were divided into two groups which received glass cannula and adhesive bandages enswathement method, respectively. Results The result showed that 0. 7% and 1% sodium pentoharhital anesthesia duration was too short, 1.5% pentoharhital sodium easily made animal deaths, and 10% chloral hydrate used for anesthesia in mice was appropriate. And the glass cannula was more suitable than adhesive bandages for enswathement. Conclusion 10% chloral hydrate anesthesia and glass cannula enswathement are availability for mouse tail skin transplatation. The tailure rate of this method is low. It is suitable for students experiment.