| 微孔板杂交法测定郴毒假单胞酵米面亚种的ＤＮＡ—ＤＮＡ同源性 |
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| 引用本文： | 焦振泉,杨瑞馥,等.微孔板杂交法测定郴毒假单胞酵米面亚种的ＤＮＡ—ＤＮＡ同源性[J].微生物学报,2001,41(1):70-75. |
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| 作者姓名： | 焦振泉 杨瑞馥 |
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| 作者单位： | [1]中国预防医学科学院营养与食品与卫生研究所，北京１０００５０ [2]中国军事医学科学院微生物与流行研究所，北 |
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| 基金项目： | 国家自然科学基金资助项目(39570619) |
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| 摘 要： | 采用一种新的ＤＮＡ－ＤＮＡ发校方法－微孔板法对郴毒假单胞菌酵米面亚种及伯克霍尔德氏菌属中１１个的ＤＮＡ－ＤＮＡ同源性进行测定。结果发现郴毒假单胞菌酵米面亚种与唐菖蒲伯克霍尔德氏菌及椰毒伯克霍尔德氏菌的ＤＮＡ－ＤＮＡ同源性均大于７５％，建议这３个种应合并为同一个种，命名为唐菖蒲的伯克霍尔德氏菌（Brukholderia gladioli)。
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| 关 键 词： | 郴毒假单胞酵米面亚种 DNA－DNA同源性 微孔板法 |
| 文章编号： | 0001-6209(2001)01-0070-06 |
| 修稿时间： | 1999年12月29 |
DETERMINATION OF DNA-DNA HOMOLOGY AMONG PSEUDOMONAS COCO VENENANS SUBSP. FARINOFERMENTANS WITH MICRODILUTION PLATE HYBRIDIZATION METHOD |
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| Z Jiao,X Liu,R Yang,Z Meng.DETERMINATION OF DNA-DNA HOMOLOGY AMONG PSEUDOMONAS COCO VENENANS SUBSP. FARINOFERMENTANS WITH MICRODILUTION PLATE HYBRIDIZATION METHOD[J].Acta Microbiologica Sinica,2001,41(1):70-75. |
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| Authors: | Z Jiao X Liu R Yang Z Meng |
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| Institution: | Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China. |
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| Abstract: | The DNA-DNA homologies among Pseudomonas cocovenenans subsp. farinofermentans and 11 species in genus Burkholderia were determined with microdilution plate hybridization method. The results showed: the homologies among P. cocovenenans subsp. farinofermentans, B. gladioli and B. cocovenenans were all over 75%. It was advised that these three species were synonym, and should be renamed as Burkholderia gladioli. |
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| Keywords: | Pseudomonas cocovenenans subsp farinofermentans DNA\|DNA homology Microdilution plate method |
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