Construction of hybrid promoters of caulimoviruses and analysis of their activity in transgenic plants |
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| Authors: | B. R. Kuluev A. V. Knyazev Ya. P. Lebedev A. A. Iljassowa A. V. Chemeris |
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| Affiliation: | 1.Institute of Biochemistry and Genetics, Ufa Research Center,Russian Academy of Sciences,Ufa,Russia |
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| Abstract: | By the techniques of DNA shuffling, PCR, and restriction-ligation, chimeric forms of cauliflower (Brassica oleracea) mosaic virus (CaMV), dahlia (Dahlia pinnata) mosaic virus (DMV), and carnation (Dianthus caryophillus) etching ring virus (CERV) promoters were obtained at various combinations. Twelve chimeric promoters were cloned into pCambia binary vectors comprising the reporter GUS gene, and their activities in transgenic tobacco (Nicotiana tabacum) plants were determined fluorimetrically. 35S promoter and those of DMV (442 bp) and CERV (371 and 501 bp) were used as controls. Seven of analyzed promoters displayed higher and seven promoters lower activity in transgenic tobacco plants than 35S promoter. The highest activity was characteristic of natural DMV promoter, and the least one — natural CERV promoter 501 bp in size. The CERV promoter 371 bp in size was approximately similar in strength to 35S promoter. |
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