Localization of p210-related proteins in green flagellates and analysis of flagellar assembly in the green alga Dunaliella bioculata with monoclonal anti-p210 |
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Authors: | J Schoppmeier K-F Lechtreck |
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Institution: | Botanisches Institut, Universit?t zu K?ln, Cologne, DE
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Abstract: | Summary. Recently, p210 was identified as a component of the flagellar basal apparatus in the green flagellate Spermatozopsis similis. In a search for potential homologues to p210, isolated cytoskeletons of several green flagellates were probed with a monoclonal
antibody, BAS4.13, against p210. In Western blots, cross-reacting bands in the molecular-mass range of 210 kDa were detected
only in the quadriflagellate Spermatozopsis exsultans. As described earlier for S. similis, the flagellar transition region was decorated in Chlamydomonas reinhardtii and several other green flagellates, whereas in the marine alga Dunaliella bioculata the antigen was present in the proximal part of the axoneme. Double immunofluorescence of D. bioculata with an antitubulin antibody further revealed dotlike signals at sites where the probasal bodies are located. Since most
of the antigen in D. bioculata was located in the axoneme, deflagellation offered a possibility to study the kinetics of its incorporation during flagellar
regeneration. The antigen was only detected after a flagellum reached a length of 3–4 μm and its integration into the growing
flagellar proceeded from proximal to distal. A similar delay in the incorporation of the antigen was also observed during
flagellar assembly on new basal bodies during cell division. Thus, the antigen of BAS4.13 was incorporated late and from proximal
to distal into the growing flagellum. We conclude that the pace and site by which individual proteins are integrated into
the flagellum differ greatly.
Received February 18, 2002; accepted May 17, 2002; published on line October 31, 2002
RID="*"
ID="*" Correspondence and reprints: Botanisches Institut, Universit?t zu K?ln, Gyrhofstrasse 15, 50931 K?ln, Federal Republic
of Germany |
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Keywords: | : Flagellar assembly Flagellar transition region Intraflagellar transport Cilium Immunofluorescence microscopy Green alga |
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