| 阿维菌素B1a组分高产菌株的定向选育 |
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| 引用本文： | 李燕霞,乔建军.阿维菌素B1a组分高产菌株的定向选育[J].工业微生物,2008,38(1):20-23. |
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| 作者姓名： | 李燕霞 乔建军 |
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| 作者单位： | 1. 天津大学化工学院制药工程系,天津,300072;华北制药股份有限公司,石家庄,050012
2. 天津大学化工学院制药工程系,天津,300072 |
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| 摘 要： | 以阿维链霉菌(Streptomyces avermitilis)1-17为出发菌株,分别使用紫外线及亚硝基胍并结合L-异亮氨酸诱导手段进行诱变处理,得到AVMB1a组分摇瓶发酵水平较出发菌株提高12.86%的突变株3-6.传代实验表明该菌株的高产性能稳定.结果表明,采用UV、NTG诱变结合L-Ile诱导的手段可以获得B1a组分显著提高的菌株.
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| 关 键 词： | 阿维链霉菌 阿维菌素B1a组分 诱变 定向选育 |
Directional screening of high producing strain of avermectin B1a component |
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| LI Yan-xia,QIAO Jian-jun.Directional screening of high producing strain of avermectin B1a component[J].Industrial Microbiology,2008,38(1):20-23. |
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| Authors: | LI Yan-xia QIAO Jian-jun |
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| Abstract: | Streptomyces avermitilis strain 1-17 producing avermectin was mutated with UV and NTG on spores, then induced and selected by L-Ile, an avermectin B1a high-yielding strain 3-6 was selected, which produced 12.86% more avermectin B1a than the parent strain. Subculture test indicated that the heredity characteristics of strain 3-6 was stable. The results showed that it was an effective way to obtain high-producing strain of averrnectin B1a component by using UV, NTG as mutagen and L-Ile as inducer. |
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| Keywords: | Streptomyces avermitilis avermectin B1a component mutagenesis screening |
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