Structural analysis of the curdlan-like exopolysaccharide produced by Cellulomonas flavigena KU |
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Authors: | Kenyon W J Buller C S |
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Institution: | (1) Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA, US |
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Abstract: | Cellulomonas flavigena KU produces large quantities of an insoluble exopolysaccharide (EPS) under certain growth conditions. The EPS has previously
been shown to be a glucose polymer and to have solubility properties similar to curdlan, a β-1,3-D-glucan produced by Alcaligenes faecalis var. myxogenes 10C3K. Furthermore, EPS purified by alkaline extraction stains with aniline blue, a dye specific for curdlan-type polysaccharides.
However, EPS-producing colonies of C. flavigena KU do not stain on aniline blue agar as do those of curdlan-producing bacteria. These facts prompted a more thorough structural
analysis of the EPS. Here we report that purified EPS is indeed identical to curdlan in primary structure, but that the native
form of the EPS may differ from curdlan in physical conformation. Journal of Industrial Microbiology & Biotechnology (2002) 29, 200–203 doi:10.1038/sj.jim.7000277
Received 19 February 2002/ Accepted in revised form 20 May 2002 |
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Keywords: | : aniline blue Cellulomonas curdlan exopolysaccharide glucan |
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