小麦淀粉粒束缚淀粉合成酶基因多态性的分子鉴定

运用6％的SDS-PAGE对14个小麦品种成熟籽粒Wx蛋白的多态性进行了鉴定，结果表明，14个小麦品种根据其Wx蛋白的缺失情况可分为6种组合类型。另外，根据Wx-A1、Wx-D1和Wx-D1这3个位点基因序列和变异情况分别设计了PCR引物，扩增结果表明：Wx-A1位点突变材料扩增产物为327bp，正常材料中扩增不到该特异带；在Wx-B1位点扩增出187bp目标带，突变材料没有该扩增产物；在Wx-D1位点扩增出约700bp目标带，突变材料没有该特异带。与前人的研究结果相比，Wx-B1引物在3个位点的扩增产物长度更短，差异更大，在2％琼脂糖胶上即可清楚分开，缩短了鉴定时间，提高了效率，为大规模筛选优质面条小麦品种提供了可能。

Molecular Identification of Wheat Granule Bound Starch Synthase Gene Polymorphism

Fourteen wheat cultivars were identified into six types of Wx proteins combinations using 6% SDS-PAGE.PCR primers were designed according to the three Wx genes sequences and their mutants,respectively.A 327 bp-band was amplified from the Wx-A1 mutant,while the band was absent for the normal alleles at the Wx-A1 locus,as well as the presence or absence of a 187 bp PCR fragment at the Wx-B1 locus and a 700 bp PCR fragment at the Wx-D1 locus,respectively,corresponding to the normal and mutant alleles.Compared with the former studies,shorter and more different PCR products at three loci,amplified by the primers designed for Wx-B1 gene can be separated in 2% agarose gel,which enables screening breeding lines for noodle use faster and effectively.