Development and scale-up of a fed-batch process for the production of capsular polysaccharide from Haemophilus influenzae |
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Authors: | Merritt J Allard G O'Toole L Swartz R Licari P |
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Affiliation: | University of Massachusetts Lowell, Department of Chemical and Nuclear Engineering, 1 University Avenue, Lowell, MA 01854, USA. |
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Abstract: | The production of the capsular polysaccharide, polyribosylribitolphosphate, from Haemophilus influenzae type b is important for the production of effective conjugate vaccines. Factors limiting the production of this polysaccharide from H. influenzae type b in liquid culture were investigated. A fed-batch fermentation was developed that increased cell density and PRP titer approximately four fold when compared to the batch fermentation. This fed-batch process was successfully scaled from the 1.5 l development scale to the 500 l manufacturing scale. The maximum cell density in the 500 l fermentation was 6 g dry cell weight per liter and the PRP concentration was 1.3 g l(-1). |
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