二斑叶螨对阿维菌素、哒螨灵和甲氰菊酯的抗性选育及其解毒酶活力变化

在室内模拟田间药剂的选择压力，用阿维菌素、哒螨灵和甲氰菊酯对二斑叶螨Tetranychuc urticae逐代处理，以选育其抗性种群。选育至12代，对阿维菌素抗性增长到6.72倍，对哒螨灵抗性增长到12.1倍，对甲氰菊酯抗性增长到19.9倍。酶抑制剂和离体酶活性的测定结果表明，阿维菌素抗性种群的多功能氧化酶和谷胱甘肽S-转移酶的活性均有所提高；二斑叶螨对哒螨灵的抗性可能与多功能氧化酶、羧酸酯酶的活性增强有关；而羧酸酯酶、多功能氧化酶和谷胱甘肽S-转移酶活性的增强可能是二斑叶螨对甲氰菊酯产生抗性的主要原因。

Resistance selection by abamectin,pyridaben and fenpropathrin and activity change of detoxicant enzymes in Tetranychus urticae

The selection for resistance by abamectin, pyridaben and fenpropathrin to Tetranychuc urticae were carried out in laboratory, as well as activity change of detoxicant enzymes. The susceptible population of T. urticae was separately treated with abamectin, pyridaben and fenpropathrin imitating field chemicals selection pressure in order to breed its resistance. The results showed that the resistance of T.urticae had reached 6 72 fold to abamectin, 19 9 fold to fenpropathrin and 12 1 fold to pyridaben after 12 generations treatment. The resistance mechanism was evaluated by activity measurement of enzymic inhibitors and detoxicant enzymes. It was concluded that the rise of resistance to abamectin was mainly associated with increased activities of mixed function oxidases and glutathione S transferase; the resistance to pyridaben was attributed to increased activities of carboxylesterase and mixed function oxidases; the main cause of resistance to fenpropathrin was the increased activities of carboxylesterase and glutathione S transferase.