Structural analysis of point mutations in the Hairless gene and their association with the activity of the Hairless protein |
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Authors: | Paz-Gómez Daniel Baizabal-Aguirre Víctor Manuel Valdez-Alarcón Juan José Cajero-Juárez Marcos Nagel Anja C Preiss Anette Maier Dieter Bravo-Patiño Alejandro |
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Institution: | Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Km. 9.5, Carretera Morelia-Zinapécuaro, C.P. 58893, La Palma, Tarímbaro, Michoacán, Mexico. |
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Abstract: | The Notch signaling pathway (NSP) is an important intercellular communication mechanism that regulates embryo development and adult physiological functions. The Hairless (H) protein is a powerful antagonist of the NSP by its interaction with the Suppressor of Hairless (SuH]) protein, recruiting the corepressors Gro and CtBP. In the present work, we examined the role of several important amino acids in different H protein domains analyzing four mutant lines of Drosophila melanogaster. The mutant alleles were evaluated by single-strand conformational polymorphism (SSCP) analysis and we located mutated regions at different positions along the sequence of the Hairless gene. |
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