Apoptosis induction in HL-60 cells and inhibition of topoisomerase II by triterpene celastrol |
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Authors: | Nagase Masahiro Oto Jinsei Sugiyama Sin Yube Kouichi Takaishi Yoshihisa Sakato Nobuo |
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Institution: | Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Japan. nagase@ag.kagawa-u.ac.jp |
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Abstract: | Celastrol, which is a triterpene purified from Celastraceae plants, has anticancer and anti-inflammatory activities. In this study we investigated to clarify whether celastrol can induce apoptosis in a human leukemia HL-60 model system. Celastrol was found to induce apoptosis, and the rank order of the potency of celastrol and its derivatives to induce internucleosomal DNA fragmentation was found to be celastrol>cela-H>the other derivatives=vehicle control. Many anticancer agents are known to possess the ability to inhibit topoisomerase II, so the inhibitory activities of celastrol and its derivatives on topoisomerase II were also explored. The rank order of the inhibitory activity was found to be celastrol>etoposide>cela-H, indicating that the apoptosis-inducing activities of cela derivatives correspond to their inhibitory activities on topoisomerase II. These data suggested that celastrol may cause its effects such as anticancer activity by the mechanism of apoptosis along with topoisomerase II inhibition. |
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