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Channel-forming (Porin) activity in Herpetosiphon aurantiacus Hp a2 |
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| Authors: | Harwardt Rainer Maier Elke Reichenbach Hans Weckesser Jürgen Benz Roland |
| Affiliation: | Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Freiburg,1. Lehrstuhl für Biotechnologie, Biozentrum der Universität Würzburg, Würzburg,2. Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany3. |
| Abstract: | Detergent extracts of cell envelopes of the gliding bacterium Herpetosiphon aurantiacus formed channels in lipid bilayers. Fast protein liquid chromatography across a HiTrap-Q cation-exchange column demonstrated that a 45-kDa protein forms the channel. The observation of a channel-forming protein suggests that Herpetosiphon aurantiacus Hp a2 has a permeability barrier on its surface. |
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