The separation of ferritin and haemosiderin for studies in the metabolism of iron |
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Authors: | J. W. Drysdale and W. N. M. Ramsay |
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Affiliation: | Department of Biochemistry, University of Edinburgh |
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Abstract: | 1. Ferritin and haemosiderin in diluted tissue cannot be quantitatively separated by centrifugation unless the pH is brought below 6. 2. These iron-storage substances can be separated from one another and from haemoglobin by ion-exchange chromatography on CM-cellulose, when good recoveries of total tissue iron are obtained. 3. The ferritin fraction has been identified by its solubility, its appearance under the electron microscope, crystallization with Cd2+ and the fact that it corresponds quantitatively to the heat-stable portion of the tissue non-haem iron. |
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