Characterization of methane oxidation by a methanotroph isolated from a landfill cover soil, South Korea |
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Authors: | Lee Eun-Hee Yi Taewoo Moon Kyung-Eun Park Hyunjung Ryu Hee Wook Cho Kyung-Suk |
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Institution: | Department of Environmental Science and Engineering, Ewha Womans University, Seoul 120-750, Korea. |
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Abstract: | A methane-oxidizing bacterium was isolated from the enriched culture of a landfill cover soil. The closest relative of the isolate, designated M6, is Methylocystis sp. Based on a kinetic analysis, the maximum specific methane oxidation rate and saturation constant were 4.93 mmol·g--dry cell weight--1·h?1 and 23 microM, respectively. This was the first time a kinetic analysis was performed using pure methanotrophic culture. The methane oxidation by M6 was investigated in the presence of aromatic (m- and p-xylene and ethylbenzene) or sulfur (hydrogen sulfide, dimethyl sulfide, methanthiol) compounds. The methane oxidation was inhibited by the presence of aromatic or sulfur compounds. |
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