Relative susceptibility of <Emphasis Type="Italic">Spodoptera litura</Emphasis> pupae to selected entomopathogenic fungi |
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Authors: | Rajesh Anand Birendra Prasad Bhupendra Nath Tiwary |
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Institution: | (1) Microbial & Molecular Genetics Lab, Department of Botany, Patna University, Patna, 800 005, India;(2) School of Biotechnology, Guru Ghasidas University, Bilaspur, Chattisgarh, 495 009, India |
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Abstract: | The pupae of Spodoptera litura (Fab.), (Lepidoptera: Noctuidae), a polyphagous pest affecting common crops in Indian subcontinent, were treated with different
concentrations of conidia of four isolates of entomopathogenic fungi belonging to three species, Metarhizium anisopliae var. anisopliae (Metschnikov) Sorokin (ARSEF 7487), Lecanicillium muscarium (Petch) Zare & W Gams (two isolates ARSEF 7037 and ARSEF 6118) and Cordyceps cardinalis Sung & Spatafora (ARSEF 7193) under laboratory conditions. Suspensions (108/ml) of conidia harvested from Sabouraud dextrose agar yeast extract (SDAY) plates resulted in the highest mortality (85.8%)
with M. anisopliae and the lowest mortality (57.3%) with C. cardinalis. The values of LC50 and LC90 suggested that M. anisopliae was the most virulent fungal strain followed by L. muscarium (ARSEF 7037). However, C. cardinalis was the least virulent species among the fungi used in the bioassay. In soil bioassays, drenching the soil with conidial
suspensions of ARSEF 7487 and ARSEF 7037 (108 conidia/g of soil) reduced the adult emergence from pupa by 81.3% and 72.5%, respectively, while premixing the sterile soil
with conidia killed lesser number of pupae (62.9% by ARSEF 7487 and 54.6% by ARSEF 7037). Our findings suggest that M. anisopliae (ARSEF 7487) and L. muscarium (ARSEF 7037) are potent entomopathogens and could be developed into biocontrol agents against rice cutworm in IPM programs.
Handling editor: Helen Roy |
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Keywords: | Bioassay Biocontrol Lecanicillium muscarium Metarhizium anisopliae Pupae Spodoptera litura |
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