Some neurohistochemical properties of nerve elements in myenteric plexus of rabbit ileum: similarities and dissimilarities to the rodent pattern |
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Authors: | R Gábriel István Pásztor Viktoria Dénes Márta Wilhelm |
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Institution: | (1) Department of General Zoology and Neurobiology, Janus Pannonius University, Pécs, Hungary, HU;(2) Department of Pharmacology and Pharmacotherapy, University Medical School, Pécs, Hungary, HU |
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Abstract: | Enteric neurons have distinct neurochemical codings in each species. The basal tone of the gastrointestinal tract of the rabbit
is low and produces neurally evoked pendular movements. Therefore, it might have an innervation pattern different from that
of other laboratory animals. We have characterised myenteric neuron populations in rabbit ileum with neurochemical markers
that are known to be associated with distinct cell types and/or fibre systems in the myenteric plexus. The density of nerve
cells estimated with the NADH-diaphorase technique was about 2500 cells/cm2 and most, if not all, neurons contained microtubule-associated protein 2. NADPH-diaphorase-positive cells were numerous.
One cell type was large and emitted long straight processes, whereas small cells bore thin filamentous dendrites. Neurons
immunoreactive for 28-kDa calcium-binding protein were rare. Over 70% of them had very strongly labelled lamellar dendrites.
Their axons were beaded and formed pericellular baskets around unstained somata. We found very few small tyrosine-hydroxylase-positive
cells. The fibre network in the plexus was very strong; the axons formed many pericellular baskets. In double labelling studies,
no co-localisation was revealed between the 28-kDa calcium-binding protein and NADPH-diaphorase. Some fibres containing 28-kDa
calcium-binding protein formed only a few contacts on somata of NADPH-diaphorase-positive cells. None of the NADPH-diaphorase-labelled
cells were found to be stained for tyrosine hydroxylase. Tyrosine-hydroxylase-positive fibres rarely made pericellular baskets
on the surface of NADPH-diaphorase-positive somata. Strongly immunolabelled pericellular baskets were never observed around
NADPH-diaphorase-positive cell somata. The results suggest that myenteric neurons in rabbit comprise distinct and characteristic
neurochemical properties that are different from the rodent pattern. Therefore, the explanation of the motility pattern of
rabbit intestine can be approached on a chemical neuroanatomical basis.
Received: 6 August 1997/Accepted: 8 October 1997 |
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Keywords: | Small intestine Enteric nervous system Neurohistochemistry Neurochemical coding Calcium-binding proteins Neurotransmitters Rabbit |
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