On the mechanism of a 60-Hz electric field induced growth reduction of mammalian cellsin vitro |
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Authors: | Mitra Azadniv Morton W Miller Christopher Cox Fredrick Valentine |
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Institution: | (1) Department of Biophysics, Division of Biostatistics, School of Medicine and Dentistry, The University of Rochester, 14642 Rochester, NY, USA;(2) Department of Forest Biology, College of Environmental Science and Forestry, 13210 Syracuse, NY, USA |
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Abstract: | Data on 60-Hz electric field (EF) induced reduction in growth rate of plant roots have strongly supported the hypothesis that the effect is related to an EF-induced transmembrane potential (V
i
m). An investigation was undertaken to determine if this hypothesis is also applicable to 60-Hz EF-induced reductions in growth rate of mammalian cells in vitro. Human lymphoblastic (RPMI 1788) and human carcinoma (HeLa) cells were selected for study, the former having a relatively small diameter (11.2 m), and the latter having a relatively large diameter (15.4 tm). The 60-Hz EFs ranged from 430–1200 V/m in the culture medium. The growth rate of RPMI 1788 cells after 4-days was depressed by about 42% at a 60-Hz EF of 1000–1200 V/m with a response threshold occurring at 950 V/m; theV
i
m at the response threshold was 8 mV There was no 60-Hz EF-induced effect on HeLa cell growth rate of aV
i
m of 8 mV (60-Hz EF=700 V/m); a statistically significant effect was achieved atV
i
m of 11 mV (950 V/m). The data support the hypothesis that above a threshold 60-Hz EF,V
i
m acts as the initial signal leading to growth rate reductions. |
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