Detection of a new polymorphism of the human prothrombin (F2) gene by combination of PASA and mutated primer-mediated PCR-RFLP |
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| Authors: | Hiroyuki Iwahana Noriko Mizusawa Katsuhiko Yoshimoto Mitsuo Itakura |
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| Institution: | (1) Otsuka Department of Clinical and Molecular Nutrition, School of Medicine, The University of Tokushima, 770 Tokushima, Japan |
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| Abstract: | A new polymorphism of the human prothrombin (F2) gene was detected by a combination of polymerase chain reaction (PCR) amplification of specific alleles (PASA) and mutated primer-mediated PCR restriction fragment length polymorphism (PCR-RFLP). The method is simple and useful for detecting polymorphisms and mutations. The new polymorphism of C1 and C2 examined by this method is highly heterozygous and serves as a good human DNA marker. |
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