Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling |
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| Authors: | Heinze Roger J Sekerina Svetlana Winkler Ines Biertümpfel Christian Oretskaya Tatiana S Kubareva Elena Friedhoff Peter |
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| Institution: | Institute for Biochemistry, FB 08, Justus Liebig University, Heinrich-Buff-Ring 58, D-35392 Giessen, Germany. |
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| Abstract: | The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS-DNA complexes and will enable their structural and functional analysis. |
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