| GM_1激活环核苷酸磷酸二酯酶的作用 |
| |
| 引用本文： | 刘飞,黄树模,徐慧君.GM_1激活环核苷酸磷酸二酯酶的作用[J].中国生物化学与分子生物学报,1997,13(1):87-90. |
| |
| 作者姓名： | 刘飞 黄树模 徐慧君 |
| |
| 作者单位： | 南通医学院生化教研室，解剖教研室!南通，226001，南通医学院生化教研室，解剖教研室!南通，226001，南通医学院生化教研室，解剖教研室!南通，226001 |
| |
| 摘 要： | 神经节苷脂GM1能激活CaM依赖性环核苷酸磷酸二酯酶，其AC50为1．56μg／ml．这种作用并不一定依赖Ca2＋的存在．在有Ca2＋存在时，GM1对PDE的最大激活活性低于CaM，仅为CaM的80．4％；没有Ca2＋存在时，GM1与CaM对PDE有同样的最大激活活性（100％）．GM1能使CaM对PDE的激活曲线左移，AC50降低，但不改变CaM对PDE的最大激活活性．三氟啦嗪能使GM1激活的PDE的活性降低，其IC50为16．3μmol／L．
|
| 关 键 词： | 神经节苷脂GM_1 钙调素依赖性环核苷酸磷酸二酯酶 三氟啦嗪 |
| 收稿时间： | 1997-02-20 |
Effect of GM_1 on Cyclic Nucleotide Phosphodiesterase |
| |
| Liu Fei,Huang Shu-Mo,Xu Hue-Jun.Effect of GM_1 on Cyclic Nucleotide Phosphodiesterase[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(1):87-90. |
| |
| Authors: | Liu Fei Huang Shu-Mo Xu Hue-Jun |
| |
| Institution: | (Department of Biochemistry, Biochemistry,Department of Anatomy,Nantong , Nantong 226001 |
| |
| Abstract: | Ganglioside GM1 can activate calmodulin dependent cyclic nucleotide phosphodiesterasein the presence or absence of Ca2＋. The AC50 of GM1 on PDE is 1. 56 μg/ml. The highest activityof PDE stimulated by GM1 is the same as CaM and is 80. 4 % of PDE activated by CaM in the absence of Ca2＋. In the presence of GM1, the activity curve of CaM on PDE moved to the left, theAC50 of CaM on PDE is decreased and the concentrations of CaM at the highest activity of PDE isalso decreased. TFP can antagonize the GM1 to stimulate PDE. The IC50 of TFP on GM1 is 16. 3μmol/ L. |
| |
| Keywords: | Ganglioside GM_1 Cyclic nucleotide phosphodiesterase Trifluoperazine |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
