Metalloproteomics: high-throughput structural and functional annotation of proteins in structural genomics |
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Authors: | Shi Wuxian Zhan Chenyang Ignatov Alexander Manjasetty Babu A Marinkovic Nebojsa Sullivan Michael Huang Raymond Chance Mark R |
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Institution: | New York Structural GenomiX Research Consortium, Bronx, New York 10461, USA. |
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Abstract: | A high-throughput method for measuring transition metal content based on quantitation of X-ray fluorescence signals was used to analyze 654 proteins selected as targets by the New York Structural GenomiX Research Consortium. Over 10% showed the presence of transition metal atoms in stoichiometric amounts; these totals as well as the abundance distribution are similar to those of the Protein Data Bank. Bioinformatics analysis of the identified metalloproteins in most cases supported the metalloprotein annotation; identification of the conserved metal binding motif was also shown to be useful in verifying structural models of the proteins. Metalloproteomics provides a rapid structural and functional annotation for these sequences and is shown to be approximately 95% accurate in predicting the presence or absence of stoichiometric metal content. The project's goal is to assay at least 1 member from each Pfam family; approximately 500 Pfam families have been characterized with respect to transition metal content so far. |
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