| Abstract: | The mode of action of acetylcholine (ACh) and succinylcholine (SCh) on the isolated frog's muscle spindle has been studied. Receptor afferent nervous supply was maintained; the appropriate spinal roots were dissected for stimulating motor axons and recording from sensory fibres. Excitatory effects on the afferent activity, when the receptor was held still and during stretching, were found with ACh or SCh concentrations of 10(-8) to 10(-3); 10(-6) g/ml being usually effective. These effects are similar to those obtained by stimulating fusimotor nerve fibres. The contractile activity of intrafusal muscle fibres which occurred during these effects was observed. Seldom, and only for high concentrations of ACh and SCh, a decrease in afferent activity following the excitatory effects was found. Tubocurarine chloride (10(-5)-10(04) g/ml) in the bath prevented both motor fibres and drugs effects. Sometimes slight transient excitation occurred at very high concentrations of the two tested substances; however, this effect was prevented by stronger curarization. The observed blocking effects were always reversed by removing tubocurarine from the bath. No more excitatory effects by motor fibres stimulation and by ACh and SCh action could be found after destruction of intrafusal muscle fibres, by pinching them as close as possible to the ends of the spindle. It is suggested that ACh and SCh act indirectly by causing mechanical changes in intrafusal muscle fibres, and that a direct action on sensory nerve endings, if any, cannot, by itself, increase the afferent activity of the receptor. |
